OcellO exploits unique technology that enables screening of molecules in human tissues cultured in 3D

high throughput

OcellO's screening platform is fully scalable with automated liquid handling, imaging and analysis in a standard 384-well format, keeping throughput up and costs low

cultured human tissues

3D cultured tissues show increased differentiation and functional properties compared to 2D cultures

compound profiling

600 features are measured to generate a detailed profile of the phenotype. Multiparametric analysis allows stringent selection of hits and also allows treatments to be compared, clustered and ranked.

compound ranking

phenotypic profiles of compounds are compared to those of reference molecules and ranked according to similarity

lead characterisation

EC50's are determined and phenotypes are evaluated for possible off-target effects

proliferation and invasion

cancer assays: multiparametric analysis is used to quantify invasion and proliferation of tumour cells, allowing a sensitive assessment of efficacy of anti-cancer compounds

Why use OcellO's 3D platform to screen and characterise your compounds?

assays for a diverse range of targets
determine activity AND specificity in a single assay
phenotypic screening in a more physiologically relevant system
parallel screening in micro-tissues derived from multiple cell lines
robust assays, highly automated and scalable to high throughput
per-well costs similar to conventional 2D assays
rapid assay development tailored for target or disease of interest

Ê3D tissue architecture and microenvironment better simulate in vivo situation
Êmulti-parametric profiling gives insight into activity and target class of uncharacterised compounds
Êmicro-tissues can be generated from diverse cell lines and screened to identify optimal target activity
Êdiverse range of specific targets can be assayed 
Êmulti-parametric analysis provides efficacy, specificity and toxicity data from a single assay to allow early-stage compound selection and ranking
Êrobust assays, highly automated and scalable to high throughput
Êper-well costs similar to conventional 2D assays
Êrapid assay development tailored for target of interest

Visualising Cancer in vitro: highly invasive prostate cancer cells proliferate and invade throughout the 3D matrix environment, but form benign spheroids when treated with the anti-cancer drug, dasatanib. Such a profound transition in phenotype is not observed in 2D culture. This rich image information is captured, quantified and used to profile compound effects

Home	Services	About OcellO
Contact	News	Careers